Fig. 3: DNAJB6b transiently converts between the three different states.
Figure depicting the transitions between the open, closed, and extended states of DNAJB6b. Two-headed arrows summarize the interactions between the domains as observed in the contact maps (see Fig. 2). The distinction between the open, closed, and extended states of DNAJB6b is made on the basis of the number of contacts between the different regions (see “Analysis of the MD simulations" in the Methods section). In all three states, anchor regions 1 and 2 are present. Anchor region 1 (AR1) consists of interactions between helix V of the G/F1 domain and helices II/III of the J-domain, forming the autoinhibitory state of DNAJB6b. Anchor region 2 (AR2) denotes the interactions between residues 118–125 of the G/F2 domain and residues 10–30 of the J-domain. These are the only interdomain contacts in the extended state. In the open state, we have additional contacts between the G/F2 and the S/T domain, and in the closed state, we have additional contacts of the CTD with the G/F1 and S/T domains. The straight gray arrows denote the interconversions between the three states, and the dashed gray arrows denote the transitions from the extended state to the near-closed and open states.
