Fig. 2: Let-7 miRNAs enable the identification of immune-skewed imMKCLs.

a Heterogenous responsive activities in imMKCLs were identified with let-7a-5p or let-7g-5p miRNA switches, resulting in let-7 low-responsive and let-7 high-responsive subpopulations. b A schematic illustration of the bulk RNA-seq sampling workflow. Let-7a-5p and let-7g-5p switches were transfected into imMKCLs, followed by the sorting of let-7 low- and high-responsive cells by flow cytometry. Three distinct imMKCL clones (clone 7, clone 7–3, M35-1) were employed for the bulk RNA-seq analysis. Gene set enrichment analysis (GSEA) were performed to compare let-7 low (n = 6 independent biological samples) and let-7 high (n = 6) subpopulations. Bar graphs showing the GSEA results of the top enriched immune-related gene sets in let-7 low-responsive imMKCLs in the proliferation phase (c) and maturation phase (d). GSEA plots showing typical enriched immune-related gene sets in the proliferation phase (e) and maturation phase (g). Representative enrichment plots from each group are displayed with the normalized enrichment score (NES), the determined nominal (non-adjusted) p-value, and the false discovery rate (FDR) derived from GSEA software. Heatmaps of the top differentially expressed TNF targets of let-7 low- or high-responsive imMKCLs in the proliferation phase (f) and maturation phase (h). Source data are provided as a Source Data file.