Fig. 3: Single-cell RNA-seq reveals the presence of transcriptionally distinct immune-skewed subpopulations that are abundant in let-7a-5p low-responsive imMKCLs. | Nature Communications

Fig. 3: Single-cell RNA-seq reveals the presence of transcriptionally distinct immune-skewed subpopulations that are abundant in let-7a-5p low-responsive imMKCLs.

From: A let-7 microRNA-RALB axis links the immune properties of iPSC-derived megakaryocytes with platelet producibility

Fig. 3: Single-cell RNA-seq reveals the presence of transcriptionally distinct immune-skewed subpopulations that are abundant in let-7a-5p low-responsive imMKCLs.

a Visualization of imMKCLs (clone 7) by uniform manifold approximation and projection (UMAP) and colored according to let-7a-5p high/low-responsive imMKCLs and imMKCL sub-clusters. b The distribution of let-7a-5p low- and high-responsive cells in each cluster, and a bar plot showing the distribution of let-7a-5p low- and high-responsive cells in each cluster. c The relative expression level of the top ten DEGs in each cluster. d The representative terms of GO:BP enriched in each cluster. GO terms under the biological process category for upregulated differentially expressed genes (DEGs) in each cluster were analyzed by g:Profiler, p-values were determined by a default hypergeometric test and correction for multiple testing has been performed by the g:SCS algorithm. e Typical immune-related GO:BP terms in each cluster. f Violin plots showing the expression level of typical thrombopoiesis-related and immune-related genes in each cluster. Source data are provided as a Source Data file.

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