Fig. 1: FISHnCHIPs schematic.
From: Highly sensitive spatial transcriptomics using FISHnCHIPs of multiple co-expressed genes
a Cell-by-gene count matrix from single-cell RNA sequencing (scRNA-seq) can be used to cluster cell types, which are characterized by their unique gene expression profiles (red cells express genes A–D; green cells express genes E–I). b Genes that are co-expressed with each other are spatially co-localized in the same cells within a tissue. By designing fluorescently labeled oligonucleotide probes to target a large set of co-expressed transcripts, FISHnCHIPs can improve the sensitivity of fluorescence detection. c Combined with repeated rounds of hybridization and washing, FISHnCHIPs enables robust and scalable mapping of cell types in tissue samples.
