Fig. 2: Experimental identification of G4-RBPs. | Nature Communications

Fig. 2: Experimental identification of G4-RBPs.

From: Predicting nuclear G-quadruplex RNA-binding proteins with roles in transcription and phase separation

Fig. 2

a Western blot for NCL after pulldown of NCL from nuclear extract with G4A4 RNA, displaying n = 2 representative experiments. b Quantification of Western blot from four pulldown experiments represented as a ratio of pulldown to input protein levels, showing NCL binding is dependent on folded G4 (K+). Data are presented as mean values ± SEM of n = 4 independent experiments, with statistical significance level determined by a two-tailed paired t-test (p = 0.032). c Volcano plot for significantly enriched proteins in K+ and Li+ pulldown mass spectrometry experiment (Supplementary Data 2). Statistical significance was estimated by unpaired t-test, with p-value < 0.05 determined as significant. Source data are provided as a Source Data file.

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