Fig. 2: miR408-5p targets IAA30 and is induced by auxin in rice. | Nature Communications

Fig. 2: miR408-5p targets IAA30 and is induced by auxin in rice.

From: Switching action modes of miR408-5p mediates auxin signaling in rice

Fig. 2: miR408-5p targets IAA30 and is induced by auxin in rice.The alt text for this image may have been generated using AI.

A Gene structure of IAA30 and alignments of miR408-5p with target sites in IAA30 3′UTR and mutant 3′UTR (m3′UTR). B Validation of IAA30 as miR408-5p target through transient expression analysis in N. benthamiana leaves. Left: The constructs in A. tumefaciens transiently introduced in N. benthamiana leaves. Middle: Representative photograph of firefly luciferase fluorescence signals when the indicated construct combinations were introduced in N. benthamiana leaves. Right: Relative reporter activity in N. benthamiana leaves expressing the indicated construct combinations. Error bars indicate SD (Tukey’s honestly significant difference, P < 0.05). Quantitative reverse transcription PCR (qRT–PCR) analysis of IAA30 (C), MIR408 (D), miR408-5p (E) and miR408-3p (F) expression in rice with auxin treatment under different time. G IAA30p-GUS and MIR408p-GUS staining in 7-d-old rice plants with and without 4 h 10 μM IAA treatment. Bars = 1 cm. H The relative intensity of GUS before and after IAA treatment shown in (F). Error bars indicate SD (Student’s t-test, **P < 0.01, ***P < 0.001). Source data underlying (B–F, H) are provided as a Source Data file.

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