Fig. 5: Impaired activation and proliferation of CD8 T is mediated by monocytes and partially rescued by ABT263.
From: Senescence drives immunotherapy resistance by inducing an immunosuppressive tumor microenvironment
a GSEA of CD8 T cells from our RNA transcriptomic data using the fgsea package to identify enriched GO. n = 1421 CD8 T cells in CTL (from three different tumors), n = 1073 CD8 T cells in TBI (from 3 different tumors), n = 1072 CD8 T cells in ABT (from three different tumors). b Shown are the EnrichmentPlot with statistical information (p-value adjusted or padj, and the Normalized Enrichment Score or NES) from CD8 T cells GSEA for the selected specific GO: T cell activation and Activation of immune response. c Violin plots showing the transcriptomic expression of Ifnγ and Cd69 effector markers in CD8 T cells. FindMarkers function with Wilcoxon Rank Sum test. n = 1421 CD8 T cells in CTL (from three different tumors), n = 1073 CD8 T cells in TBI (from 3 different tumors), n = 1072 CD8 T cells in ABT (from three different tumors). d Schematic representing the ex vivo proliferative capacity of CFSE stained CD8 T cells within the TME, with or without myeloid cells (CD11b+), from the indicated groups following stimulation with activation beads. The percentage of proliferative CD8 T cells was evaluated using the FlowJo software and proliferation tools. n = 9 CTL, n = 4 CTL (-CD11b), n = 4 CTL + ABT263, n = 4 CTL + ABT263 (-CD11b), n = 11 TBI, n = 5 TBI (-CD11b), n = 10 TBI + ABT263, n = 5 TBI + ABT263 (-CD11b). Ordinary one-way ANOVA with Tukey correction. Shown is the mean ± SEM. Source data are provided as a Source Data file.
