Fig. 3: Polyamines’ ferroptosis-promoting property depends on PAOX/SMOX mediated H2O2 production.
From: Polyamine-mediated ferroptosis amplification acts as a targetable vulnerability in cancer
a Cell viability in A549 or HT1080 cells treated with RSL3 following siRNAs transfection for 48 h. b The protein levels of PAOX and SMOX in cancer cell lines with indicated genotypes determined by western blotting. c Cell viability in Cas9-NC and PAOX/SMOX-KO A549 or HT1080 cells treated with RSL3 following pre-treatment with polyamines. d Cell viability in Cas9-NC and PAOX/SMOX-KO A549 or HT1080 cells treated with RSL3 following pre-treatment with AMXT-1501 and DFMO. e Relative H2O2 level in Cas9-NC and ODC1-KO A549 or HT1080 cells treated with RSL3 (A549: 2 μM; HT1080: 0.25 μM) for 3 h, determined by H2DCFDA. f Relative H2O2 level in A549 or HT1080 cells treated with RSL3 following pre-treatment with polyamines. g Relative H2O2 level in Cas9-NC and PAOX/SMOX-KO A549 or HT1080 cells treated with RSL3. h Cell viability in vehicle- and △catalase-overexpressed A549 or HT1080 cells treated with RSL3 following pre-treatment with polyamines. i Cell viability in A549 or HT1080 cells with indicated genotypes treated with RSL3. Data are presented as the mean ± SD, n = 3 independent experiments. Unpaired two-tailed Student’s t tests are used. Source data are provided as a Source Data file.
