Fig. 4: Comparison of paternal and offspring metabolic traits accounting for individual differences in food intake.
GAM predictions of the influence of paternal daily consumption (kJ/day) of Protein and Carbohydrates, sliced through the response surface at the 3rd quartile of Fat intake, on response variables: a, b Fat mass as a percentage of body mass measured by EchoMRI at 18 weeks of age, c, d HOMA-IR calculated from an oral glucose tolerance test at 18 weeks of age. Note, offspring response surfaces are plotted against paternal intake values. Surfaces sliced through the 1st and 2nd quartile of Fat intake are shown in Fig S4. An effect of dietary macronutrient intake on the outcome of interest was inferred, when the non-null statistical model (i.e., that fitting an effect of macronutrient intake) led to a statistically significant increase in the deviance explained over the null model based on a χ2 test. a F0 % Fat Mass χ2 = 232.3, df = 10.04, p = 4.2e-8, n = 60, b F1 % Fat Mass χ2 = 22.29, df = 14.93, p = 6.8e-6, n = 50, c F0 HOMA-IR χ2 = 1.95, df = 9, p = 1.7e-5, n = 60, d F1 % HOMA-IR χ2 = 0.4, df = 9, p = 0.02, n = 51. Full output from model comparisons is provided in Source Data File 1. Source data for fathers (a, c) are provided in Source Data File 1, for female offspring (b) in Source Data File 2 and for male offspring (d) in Source Data File 3. Created with BioRender.com.
