Fig. 6: CR-Isl1 is a potential Wnt-responsive element. | Nature Communications

Fig. 6: CR-Isl1 is a potential Wnt-responsive element.

From: Tgfbr1 controls developmental plasticity between the hindlimb and external genitalia by remodeling their regulatory landscape

Fig. 6

A Schematic representation of the CR-Isl1 region of Fig. 4J. The top panel shows the ATAC-seq signal in control GT and the placental conservation score. The footprint corresponded to a ~65 bp region containing a Gata3 binding motif (red), and Lef1 binding motif (green). An additional Lef1 binding motif (blue) was identified 21 bp upstream of the Gata3 motif, although it was not included within the footprint. The genomic sequences of these motifs are shown along with position frequency matrices (PFMs). Activation of the β-galactosidase reporter by the CR-Isl1 (B), CR-Isl1DLef1 (C), and CR-Isl1DDLef1 (D) elements in E11.5 embryos. Black arrowheads show expression in otic vesicle; white arrow indicates expression in the posterior limb bud; black arrow shows expression in the GT.

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