Fig. 3: Proof-of-concept production of PHB from debranched maltodextrin by the designed ivSEB. | Nature Communications

Fig. 3: Proof-of-concept production of PHB from debranched maltodextrin by the designed ivSEB.

From: ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA

Fig. 3

a Schematic of the XPK essential pathway (i.e. without TK, TIM, ALD, FBP, TAL, RPI, and PGI), in which the consumption of 2 glucose equivalents of maltodextrin results in the generation of 1 monomer equivalent of PHB. b Schematic of the FPK essential pathway (i.e. without TK, TIM, ALD, FBP, TAL, RPI, and RPE), in which the consumption of 5 glucose equivalents of maltodextrin results in the generation of 2 monomer equivalents of PHB. c Results showing the production of PHB from around 55.6 mM glucose equivalent (10 g/L) of maltodextrin. The reactions commenced upon the addition of maltodextrin and were continuously monitored at 15 min intervals via absorbance at 600 nm (OD600) throughout the experiment. Source data are provided as a Source Data file.

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