Fig. 4: One-pot, one-step production of PHB from 100 mM debranched maltodextrin by the designed ivSEB.

Experimental data were represented as dots, while simulation results were shown as dotted lines, generated using Model 2 which had undergone two rounds of model fitting. The first round of model fitting utilized experimental data from Figs. 4a, b. The second round of model fitting used experimental data from Fig. 4a–c. a PhaA was loaded at 1 mg/mL (0.78 mU/mL) while other enzymes were loaded at 1 U/mL. b PhaA was loaded at 5 mg/mL (3.9 mU/mL) while other enzymes were loaded at 5 U/mL. c Enzymes were loaded at the suggested concentrations after the first round of in silico optimization, as summarized in Supplementary Table 6. d Enzymes were loaded at the suggested concentrations after the second round of in silico optimization, as summarized in Supplementary Table 6. Reactions were performed in triplicate (n = 3 biologically independent samples) and data are presented as mean values ± standard deviation (SD). Source data are provided as a Source Data file.