Fig. 2: Characterization and validation of non-randomly distributed low-complexity regions (LCRs) in monkeypox virus (MPXV) genome sequence 353R.

a LCR3 sequence validation using MPXV 353R nanopore sequencing data and 15 additional raw data sequencing reads downloaded from the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA). Box plots representing median (middle line), 25th and 75th percentile (box), and 5th and 95th percentile (whiskers) outliers are plotted as single dots. Blue dashed lines represent trends of LCR3 repeats in MPXV lineages (n = 16 sequences). (Wilcoxon rank-sum test p-values are indicated in the graph.) b LCR pair 1/4 sequence validation using MPXV 353R nanopore sequencing data and 20 additional raw data sequencing reads downloaded from NCBI SRA. Box plots representing median (middle line), 25th and 75th percentile (box), and 5th and 95th percentile (whiskers) outliers are plotted as single dots. Blue dashed lines represent trends of LCR1/4 repeats in MPXV lineages (n = 21 sequences). (Wilcoxon rank-sum test p-values are indicated in the graph.) Detailed information on the represented materials, along with originator and epidemiological data, is provided in Supplementary Data 6.