Fig. 1: Following YFP-MutL foci in E. coli.
a The mother machine microfluidic chip contains ~ a thousand narrow microchannels in which bacteria grow in a single file. Modified from Robert et al.17 with permission from AAAS and the authors. b Tracking replication errors with fluorescent YFP-MutL in mutH cells (depicted in red). The fluorescent YFP-MutL focus (yellow dot) remains associated with DNA until a subsequent replication cycle converts the error into a mutation. Modified from Robert et al.17 with permission from AAAS and the authors. c Representative fluorescent image (left) of mutH cells expressing YFP-MutL and the corresponding kymograph (right). One representative experiment out of 2 performed. The kymograph obtained by BACMMAN software displays the segmentation and tracking over time (time interval of 7.5 s) of cells (cyan contours) and YFP-MutL foci (magenta contours for segmentation and colored lines for tracking) within a single microchannel (gray rectangle). d Rates of YFP-MutL foci in mutH, wild-type (WT), and MF1R strain under different levels of induction of the PBAD promoter (no induction, arabinose 0.1% and 0.5%). The bars represent the average of individual experiments (dots). See the first section of Supplementary Note 1 for details, Supplementary Table 2 for the values of individual experiments, and Supplementary Table 3 for comparisons of the different conditions. e The distribution of YFP-MutL foci lifetimes of one representative experiment per condition (all experiments are shown in Supplementary Fig. 1, see also Supplementary Table 4 for the total number of experiments performed and foci analyzed). Source data are provided as a Source Data file.
