Fig. 2: Experimental approach to study the efficiency of error repair at the single-cell level.

A replication error that has been detected by MMR can either be repaired, leading to a short-lived YFP-MutL focus (bottom and yellow-shaded part of the schematic), or converted into a mutation, resulting in a long-lived focus (top and pink-shaded part of the schematic). YFP-MutL foci are represented as yellow dots in red cells. Modified from Robert et al.17 and Elez43 with permission from AAAS and the authors.