Fig. 3: Growth factor-evoked glucose uptake requires receptor/GLUT1 endocytosis.
From: Endocytic vesicles act as vehicles for glucose uptake in response to growth factor stimulation
a Serum-starved Swiss 3T3 fibroblasts were pre-treated with or without BAY876 (50 nM) and subjected to glucose uptake assay with PDGF-BB (50 ng/ml) in the presence of 2DG for 10 min. Graph shows 2DG6P normalized to total cellular proteins. Bars and error bars show means of biological replicates (deep-color dots, n = 3) and ±SD. Values of biological replicates are means of technical replicates (light-color dots). One-way ANOVA and post-hoc Tukey’s test. b siRNA-treated Swiss 3T3 fibroblasts were serum-starved and subjected to glucose uptake assay with PDGF-BB (50 ng/ml). Graph shows 2DG6P normalized to total cellular proteins. Bars and error bars show means of biological replicates (deep-color dots, n = 3) and ±SD. Values of biological replicates are means of technical replicates (light-color dots). One-way ANOVA and post-hoc Tukey’s test. c Serum-starved control or dynamin TKO MEFs were stimulated with 50 ng/ml PDGF-BB for 10 min, and immunostained with the indicated antibodies. Higher magnification images of the boxed regions are shown. Nuclei were stained with DAPI (blue). Representative data are shown from one of 2 independent experiments. Scale bar: 20 μm. d Serum-starved control or dynamin TKO MEFs were subjected to glucose uptake assay with PDGF-BB (50 ng/ml). Graph shows 2DG6P normalized to total cellular proteins. Bars and error bars show means of biological replicates (deep-color dots, n = 4) and ±SD. Values of biological replicates are means of technical replicates (light-color dots). One-way ANOVA and post-hoc Tukey’s test. e Serum-starved control or dynamin TKO MEFs were stimulated with PDGF-BB (50 ng/ml). Lysates were subjected to immunoblotting with the indicated antibodies. Representative data are shown from one of 2 independent experiments. f Serum-starved Swiss 3T3 fibroblasts were subjected to glucose uptake assay with PDGF-BB (50 ng/ml), PDGF-BB-conjugated microbeads, or unconjugated PDGF and microbeads. Graph shows 2DG6P normalized to total cellular proteins. Bars and error bars show means of biological replicates (deep-color dots, n = 3) and ±SD. Values of biological replicates are means of technical replicates (light-color dots). One-way ANOVA and post-hoc Tukey’s test. Source data are provided as a Source Data file.
