Fig. 5: Cellular carbon homeostasis in glucose-limited condition requires receptor endocytosis-dependent glucose uptake.

a Conditional Dynamin TKO MEFs treated with 4-OHT (Dynamin TKO) or untreated (control) were cultured in DMEM containing the indicated concentrations of glucose without FBS and pyruvate in the presence of PDGF-BB (20 ng/ml) for 3 days. Surviving cells were visualized by staining with crystal violet. Graph shows relative staining intensity in 3 wells (technical replicates, n = 3), with the value of control cells set as 1. Error bars represent ±SD. P values were calculated by one-way ANOVA and post-hoc Tukey’s test. Representative data are shown from one of 2 independent experiments. b Control MEFs or dynamin TKO MEFs were cultured in DMEM containing 0.1 g/ml glucose without FBS and pyruvate in the presence of PDGF-BB (20 ng/ml) for 24 h. Cellular metabolites were extracted and analyzed by mass spectrometry. Graph shows principal component analysis. c Heatmap shows unsupervised hierarchical clustering based on levels of 116 metabolites. d Graphs show average amount of each metabolite in the 3 samples (technical replicates, n = 3), adjusted by cell numbers. Error bars represent ±SD. P values were calculated using two-tailed Welch’s t tests. Scheme shows glycolytic and TCA cycle metabolites. Metabolites not tested are labeled in gray, and asterisks indicate metabolites that were not detected in any sample. e Graphs show average amount of each metabolite in 3 samples (technical replicates, n = 3), normalized to cell number. Error bars represent ±SD. P values were calculated using two-tailed Welch’s t tests. Data was obtained from a single experiment. Source data are provided as a Source Data file.