Fig. 2: Responders to fecal microbiota transplantation (FMT) have pronounced effect on differential gene expression in their colonic mucosa that is not present in non-responders.

A Volcano plot of differential gene expression in responders following treatment with FMT for 8 weeks. Total genes identified by DESeq2 to be significantly regulated n = 78 (q < 0.05). Tx0Y, responder baseline; Tx8Y, responder post-FMT. Red, significantly upregulated; Green, significantly downregulated. B Volcano plot of differential gene expression in non-responders following treatment with FMT for 8 weeks. Total genes identified by DESeq2 to be significantly regulated n = 0 (q < 0.05). Tx0N, non-responder baseline; Tx8N, non-responder post-FMT. C Significantly regulated pathways (q < 0.05) in responders following FMT treatment for 8 weeks. Regulated pathways were identified using GAGE. D Significantly regulated pathways (q < 0.05) in non-responders following FMT treatment for 8 weeks as identified by GAGE. E Top regulated genes (n = 60) in pathways comprising the largest number of genes (tight junctions; calcium signaling). Genes were classified by q value and selection corresponded to q < 0.5.