Fig. 4: Gbp5^–/– mice are more resistant to colitis than wild-type littermates.

A Schema of experimental colitis model using littermate wild-type (WT) and Gbp5^–/– mice. Mice were cohoused until 24 h prior to exposure to dextran sulfate sodium (DSS). B Change in body weight of mice throughout the 10 days of the model. Differences between groups were tested using Two-Way ANOVA with Šídák’s multiple comparisons test. Days 0–7 p > 0.8; Day 8: p = 0.044; Day 9: p = 0.0103; Day 10: p = 0.0065. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01. C Representative images of colon lengths for littermate WT and Gbp5^–/– mice. D Differences in colon lengths between littermate WT (n = 12) and Gbp5^–/– (n = 14) mice at day 10. Data distribution was assessed using the Shapiro-Wilk test and differences were tested using a two-tailed unpaired t test (t = 2.86, p = 0.0087). Data are presented as mean ± SEM. **p < 0.01 E Haematoxylin and eosin staining of the colonic tissue of mice on day 10. Data are representative of three independent experiments. Scale bars, 1000 μm (top), 100 μm (bottom). Arrowheads indicate histological damage including inflammation, ulceration and hyperplasia. F Histological score of the colonic tissue of WT (n = 12) and Gbp5^–/– (n = 14) mice on day 10 presented as mean ± SEM. Each symbol represents an individual mouse. Data distribution was assessed using the Shapiro-Wilk test and differences were tested using a two-tailed unpaired t test (t = 1.64, p = 0.11). G Histological scores of the colonic tissue of WT (n = 12) and Gbp5^–/– (n = 14) mice on day 10 stratified by region and presented as mean ± SEM. Each symbol represents an individual mouse. Differences were tested using a two-way ANOVA with Šídák’s multiple comparisons test and statistical significance in the middle region was validated with a two-sided Mann-Whitney test (U = 41.5, p = 0.024). ns, not significant; *p < 0.05. Source data are provided as a Source Data file.