Fig. 1: VG3 plexus calcium responses from the retina that was later reconstructed with electron microscopy. | Nature Communications

Fig. 1: VG3 plexus calcium responses from the retina that was later reconstructed with electron microscopy.

From: Subcellular pathways through VGluT3-expressing mouse amacrine cells provide locally tuned object-motion-selective signals in the retina

Fig. 1

a Two-photon imaging of GCamp6f expression in VG3s. Two-photon structural map of VG3 plexus shown in cyan. Transmitted light image of blood vessels in red. Averages of functional images of VG3 dendrites are shown in magenta. Single frame of calcium response to stimulus is shown in green. b Colors indicate pixels pooled for measuring calcium response at targeted points in VG3 plexus (grey). Traces show examples of four raw (red) and averaged (black) fluorescence changes in response to bar stimulus. c Colored dots show functionally characterized dendrites that could be mapped onto the 3DEM reconstruction of the VG3 plexus (gray arbors). d Histograms show ON/OFF polarities of neurites from each functional imaging plane. Colors correspond to the planes in (c). INL inner nuclear layer, IPL inner plexiform layer.

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