Fig. 2: Enzyme immobilization on nano- and isoporous BCP membranes. | Nature Communications

Fig. 2: Enzyme immobilization on nano- and isoporous BCP membranes.

From: An enzymatic continuous-flow reactor based on a pore-size matching nano- and isoporous block copolymer membrane

Fig. 2

a 3D structure of wild-type phytase (YmPh-WT) and YmPh genetically fused to the MBP LCI (YmPh-LCI). b SEM images of PS-b-P4VP isoporous membranes: top surface and cross-section. c Dose-response curve to analyze binding efficiency of YmPh-WT and YmPh-LCI on isoporous BCP membranes (@M indicates membrane-bound YmPh). Error bars represent s.d. of the mean from three independent experiments (n = 3). AFM height images of dense PS-b-P4VP films (d) treated with buffer solution as a control (Tris-HCl buffer (50 mM, pH 8.0)) and (e) after YmPh-LCI immobilization. f 3D reconstruction image from a z-stack along the cross-section of the membranes with fluorescently labelled YmPh-LCI_Cy3. The membrane’s top layer faces up. g A reconstructed elemental line scan of the gold signal along the cross-section of the membranes with gold-labelled YmPh-LCI_Au from energy-dispersive X-ray spectroscopy (EDX) mapping. The membrane’s top layer faces right. Transmission electron microscopy (TEM) bright-field images of the cross-section of the membranes with YmPh-LCI_Au: (h) low magnification, (i) high magnification. Results were reproduced three times independently; representative micrographs are shown. Source data are provided as a Source Data file.

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