Fig. 7: Persistent optogenetic activation of DRN 5-HT neuron terminals in PFC induce internal synchronized state and vigilance behavior in mice.
From: Serotonergic modulation of vigilance states in zebrafish and mice
a Drawing shows stereotaxic injection of the Cre-dependent AAV virus into the DRN, optogenetic activation of DRN 5-HT neuron terminals, in vivo EEG recording in the prefrontal cortex, and the locomotion test in mice with a timeline. b Immunofluorescence image showing co-localization of hChR2-mCherry and anti-5-HT staining of DRN 5-HT neuron terminals in mice PFC. Image represents results from eight independent experiments. c–f Representative EEG trace, heat map of EEG frequency distribution, analysis of locomotor speed, and EEG power spectra density plot (top to bottom) before, during, and after 120 s optogenetic activation of DRN 5-HT neuron terminals in Slc6a4-hChR2 mice (c) and in Slc6a4-mCherry mice (e). The dashed line rectangles outlined in (c) and (e) are shown enlarged in (d) and in (f), respectively. N = 8 mice in each group.
