Fig. 4: Biocompatibility and ex vivo therapeutics of meniscus adhesives.
From: Silk fibroin hydrogel adhesive enables sealed-tight reconstruction of meniscus tears
A Schematic illustration of co-culture with hydrogels and live/dead cell assays. B Cell viability of rabbit meniscus cells co-cultured with hydrogels within 7 days. Data are presented as mean ± SD (n = 3 independent cell experiments). C Cell Counting Kit 8 (CCK-8) assays of rabbit meniscus cells co-cultured with hydrogels within 7 days. Data are presented as mean ± SD (n = 3 independent cell experiments). D Alcian blue (AB) staining and immunofluorescence staining of meniscus cells. E Meniscus-related gene (Sox9, Col2a1, and ACAN) expression in the S-Gel and S-PIL10 at 2 weeks. Data are presented as mean ± SD (n = 3 independent cell experiments) and exact p-value was calculated with two-tailed student’s t-tests. F TFG-β1 release-time curves of S-Gel and S-PIL10. Data are presented as mean ± SD (n = 3 independent samples). G Inflammatory condition after meniscus tears and DDPH scavenging and PTIO scavenging of S-Gel and S-PIL10. Data are presented as mean ± SD (n = 3 independent hydrogels) and exact p-value was calculated with two-tailed student’s t-tests. Scale bars are 200 μm.
