Fig. 3: The GASF strategy for proteome-wide quantification of cell surface lysine reactivity. | Nature Communications

Fig. 3: The GASF strategy for proteome-wide quantification of cell surface lysine reactivity.

From: A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell

Fig. 3: The GASF strategy for proteome-wide quantification of cell surface lysine reactivity.

a Schematic illustration of the cell surface lysine reactivity profiling workflow. b Comparison of the cell surface lysines of TMPs (known topology domain information in Uniprot database) in our surfaceome data with previous whole proteome data.6 c Distribution of the SILAC-ABPP ratios for 2639 lysines definitely and potentially exposed on the cell surface from living Hela cells treated with 50 versus 500 μM OPA-S-S-alkyne. The inset shows the zoom-in view of the group of hyper-reactive lysines (R ≤ 2.0, red boxed) with representative sites marked (K285 of CD105, K224 of ROBO1, K220 of CD298, K61 of PTPRF and K382 of ROR2). d Number of hyper-reactive and quantified lysines per protein shown for proteins found to contain at least one hyper-reactive lysine. e Distribution of functional classes of TMPs that contain hyper-reactive lysines.

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