Fig. 4: Functional characterization of the hyper-reactive lysines in ROR2 and ENG. | Nature Communications

Fig. 4: Functional characterization of the hyper-reactive lysines in ROR2 and ENG.

From: A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell

Fig. 4: Functional characterization of the hyper-reactive lysines in ROR2 and ENG.

a Co-crystal structures of proteins with hyper-reactive lysines in complexes with their interacting proteins. Hyper-reactive lysines (K382 for ROR2 and K285 for ENG) are shown in red, ROR2 and ENG in green, their interacting proteins in orange (parental rabbit mAb for ROR2, and BMP9 for ENG). b Hyper-reactive lysines can be site-selectively labeled by OPA-S-S-alkyne, the labeling was blocked by mutation of the hyper-reactive lysine to arginine. HEK293T cells expressing ROR2 and ENG (and their corresponding lysine-to-arginine mutant) as Flag epitope-tagged proteins were treated with the OPA-S-S-alkyne, reacted with azide-biotin for enrichment, eluted and analyzed by western blotting using Flag antibody. Images shown are representative of three independent experiments. Source data are provided as a Source Data file.

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