Fig. 5: Comparative cellulosomal analysis between representatives of the two tortoise-affiliated genera (genus Astrotestudinimyces, strain B1.1; and genus Testudinimyces, strain T130A) and one mammalian affiliated strain (Orpinomyces joyonii strain AB3).

A Maximum likelihood mid-point rooted phylogenetic tree showing the relationship between scaffoldin ScaA protein homologs identified in Orpinomyces joyonii strain AB3 (12 copies denoted AB3_1 through AB3_12 and shown in purple text), Astrotestudinimyces strain B1.1 (2 copies denoted B1.1_and B1.1_2 and shown in brown boldface text), and Testudinimyces strain T130A (2 copies denoted T130A_and T130A_2 and shown in orange boldface text) in comparison to a reference set of 319 Neocallimastigomycota ScaA homologs retrieved from Uniprot. All reference ScaA homologs are shown with their Uniprot ID followed by the AGF strain name color-coded by genus, as shown in the legend. B Comparison of the percentage distribution of functions (as predicted by NCBI Conserved Domain database) encoded by cellulosomal peptides (all predicted peptides harboring a non-catalytic dockerin domain in the two tortoise affiliated genera (genus Astrotestudinimyces, strain B1.1; and genus Testudinimyces, strain T130A) and the mammalian affiliated strain (Orpinomyces joyonii strain AB3) and destined to the extracellular milieu (as predicted by DeepLoc)). The total number of peptides is shown above each column. C CAZyome composition of the predicted cellulosome in the three strains compared. Source data for Fig. 5B, C are provided as a Source Data file.