Table 2 Quantification of A antigen expression in control vs treated kidneys during NMP or HMP

From: Enzymatic conversion of human blood group A kidneys to universal blood group O

Time after enzyme addition (h)

Percentage of A antigen remaining during NMP

Percentage of A antigen remaining during HMP

 

Control (%)

Treated

(%)

p

Control (%)

Treated

(%)

p

1

115

[81,148]

56 [35,78]

0.0056

77

[57,98]

70

[46,95]

0.2856

2

110

[71,148]

19

[12,25]

<0.0001

54 [43,64]

23 [13,32]

0.0015

3

/

/

/

62 [34,91]

24 [11,37]

0.0047

4

115

[88,142]

25 [16,35]

<0.0001

47 [31,62]

20 [10,29]

0.0047

5

/

/

/

38 [23,52]

13 [7,21]

0.0042

6

97

[71,124]

18

[12,24]

<0.0001

85

[67,103]

21 [12,29]

<0.0001

12

/

/

/

119

[69,168]

29 [18,39]

0.0005

24

/

/

/

96

[72,120]

28

[18,38]

0.0042

  1. Values represent the mean percentage of A antigen expression in images from the specified timepoint relative to the pre-treatment biopsy in each experiment. 95% CI are shown underneath in square brackets. At each timepoint, data are pooled from a minimum of three independent kidney biopsies providing at least 18 fields of view. To compare expression in control and treated kidneys, a two-tailed Wilcoxon matched-pairs signed rank test was completed on each row, with Holm-Sidak’s multiple comparisons correction. P-values are shown to 4 decimal places. Significant results (p < 0.0500) are highlighted in bold.
  2. NMP normothermic machine perfusion, HMP hypothermic machine perfusion.