Fig. 3: Ultrasound stimulation activates TRAAK and MscS channels.
From: Tension activation of mechanosensitive two-pore domain K+ channels TRAAK, TREK-1, and TREK-2
A Schematic of recording setup for ultrasound stimulation during simultaneous patch recording and imaging. A 3.5 MHz ultrasound transducer is mounted into a custom 3D-printed recording dish and patched membranes are positioned at the position of maximum ultrasonic power. B Macroscopic currents from a TRAAK-containing patch in response to an ultrasound step protocol (Vhold = 0 mV, transducer driving voltage = 0–1 V, Δ driving voltage = 100 mV. 100 mV intervals displayed with measured power at patch position in W/cm2 indicated). C Macroscopic currents from a MscS-containing patch in response to an ultrasound step protocol (Vhold = 0 mV, transducer driving voltage = 0–0.3 V, Δ driving voltage = 50 mV, measured power at patch position in W/cm2 indicated).
