Fig. 3: Rac1, Rho1 and focal adhesions control follicle cell expansion behavior.

a–e Representative time-lapse images of WT (a), Rac1DN-expressing (b), Rho1DN-expressing (c), Paxillin-overexpressing (d) and Talin RNAi-expressing (e) follicular cell basal domains monitored by E-cadherin-GFP (seen in Supplementary Movies 4–8), showing spatiotemporal effect on cell expansion waves composed of ruffling and spreading processes. A and P represent anterior and posterior regions of egg chamber and arrows mark the wave direction. Light pink colors mark ruffling area, and different colored lines mark cell boundary at different time points, in (a-e). Dotted circles mark the whole tissue boundary, and dotted rectangles mark the enlarged area showing dynamical changes of cell expansion behavior. f Boundary outlines mark the temporal changes (color bars) of one representative WT, Rac1DN-expressing, Rho1DN-expressing, Paxillin-overexpressing, or Talin RNAi-expressing follicle cell during the ruffing (above) and spreading (below) phases. g–k Quantifications of circularity index during the ruffling phase (g), basal area during the increasing phase (h), and basal area (i), migration distance (j) and migration velocity (k) during the spreading phase in the indicated follicle cells. l-o Quantifications of circularity index during the ruffling phase (l), basal surface area (m), migration velocity (n) and migration orientation (o) during the spreading phase in the indicated follicle cells. Scale bars are 20 μm in (a–e). The middle line shows medians, upper and lower lines as 25th and 75th percentiles, each datapoint is displayed as a dot, in (g–o). All P values have been listed in Supplementary Note 1. Source data are provided as a Source Data file.