Fig. 6: Expanding properties of basal actomyosin networks.
From: Basal actomyosin pulses expand epithelium coordinating cell flattening and tissue elongation
a Basal Myosin-II images of a representative follicle cell before (panel on the left) and after ablation (indicated by the dashed line, represented by kymographs illustrating network recoil along the D–V axes after ablation) of basal actomyosin networks along the A–P axis, during pre-wave and spreading phases. Myosin-II signals have been visualized by Sqh-GFP. b Maximum recoil speed after ablations in follicle cells during the pre-wave and spreading phases. c Nanoscale fiber structures of basal actomyosin networks in follicle cells during the pre-wave and spreading phases. Dotted boxes mark the analysis region of F-actin and Myosin-II signal combination for the fiber connection state. The fiber signals of F-actin and Myosin-II have been combined to show either connected or disconnected filamentous structures during pre-wave or spreading phase. d Nanoscale structures of basal actomyosin networks in follicle cells during the indicated phases, including raw images and ROI enlarged images of F-actin and Myosin-II signals. e Representative cartoons summarizing linear and non-linear patterns of basal actomyosin networks before, during and after epithelial expansion behavior. Green and red colors mark F-actin and Myosin-II signals, respectively. f Nanoscale fiber dynamics of basal F-actin and Myosin-II signals in follicle cells during the pre-wave and spreading phases. The arrow direction indicates the signal flow orientation, and the length indicates the relative velocity. g Spatial (left) and temporal (right) correlation analyses of basal F-actin and Myosin-II signals (see Methods) in follicle cells during the pre-wave and spreading phases. Data are presented as mean values ± SD. Scale bars are 5 μm in (a, c, d, f). The middle line shows medians, upper and lower lines as 25th and 75th percentiles, each datapoint is displayed as a dot in (b). The experiments in (a, c, d, f) were repeated 10 times independently. P value in (b) have been listed in Supplementary Note 1. Source data are provided as a Source Data file.
