Fig. 2: Two RNA activators co-activate Cas13a with the assistance of an auxiliary sequence.
From: The CRISPR-Cas13a Gemini System for noncontiguous target RNA activation
a Schematic of two RNA activators co-activate Cas13a with the assistance of an auxiliary sequence. F-Q represents FAM and BHQ-labeled RNA. b Fluorescence analysis of HEPN-nuclease activity of Cas13a by two 21-, 23-, or 25-nt RNA activators. R1 and R2 are abbreviations of RNA activator 1 and RNA activator 2, respectively. c Apparent cleavage rate of two 21-, 23-, or 25-nt RNA activators-mediated Cas13a trans-cleavage reporter relative to contiguous target RNA (T20). d Typical fluorescence intensity curves in the presence of varying concentrations of 25-nt RNA activators (1–100 pM). e Derived calibration curve corresponding to the fluorescence changes at different concentrations of 25-nt RNA activators. Data are presented as mean values ± standard deviation from three independent experiments. Source data are provided as a Source data file.
