Fig. 2: Structural rearrangements of the ISCU2 assembly site upon FXN binding facilitate persulfide transfer. | Nature Communications

Fig. 2: Structural rearrangements of the ISCU2 assembly site upon FXN binding facilitate persulfide transfer.

From: Mechanism and structural dynamics of sulfur transfer during de novo [2Fe-2S] cluster assembly on ISCU2

Fig. 2

a Overlay of the (Fe-NIAU)2 and (Fe-NSIAUSX)2 structures shows the structural changes upon FXN binding (Cys381NFS1 not shown for figure clarity). While the constantly Fe2+-coordinating Cys95ISCU2 and Asp71ISCU2 hardly change positions, Cys138ISCU2 moves toward the Fe2+ and now coordinates the metal. Concomitantly, His137ISCU2 and Cys69ISCU2 are shifted away from the ISCU2 assembly site, and His137ISCU2 is now accommodated in a pocket formed by residues including Trp155FXN, Ser157FDX and Pro163FDX (b). c The pre-sulfur transfer state of the (Fe-NSIAUSX)2 structure could conceivably allow for sulfur delivery from Cys381NFS1 to both Cys95ISCU2 (3.4 Å distance) and Cys138ISCU2 (3.6 Å distance).

Back to article page