Fig. 1: The increased levels of crotonylation in human renal biopsies of CKD and fibrotic kidneys from mice. | Nature Communications

Fig. 1: The increased levels of crotonylation in human renal biopsies of CKD and fibrotic kidneys from mice.

From: Inhibition of ACSS2-mediated histone crotonylation alleviates kidney fibrosis via IL-1β-dependent macrophage activation and tubular cell senescence

Fig. 1: The increased levels of crotonylation in human renal biopsies of CKD and fibrotic kidneys from mice.The alternative text for this image may have been generated using AI.

A Representative IHC staining of pan-Kcr antibody in healthy control subjects and patients with chronic kidney diseases. Scale bar: upper panels: 50 μm; lower panels:20 μm. B Quantitative IHC analysis of pan-Kcr level in renal nuclear from control and patients with chronic kidney diseases using ImageJ 6.0 software; using Pearson correlation statistical analysis, two-sided statistical tests. (n = 6 for ANCA, n = 9 for DN, n = 3 for IgA, n = 3 for FSGS). C IF staining of H3K9cr (green) and DAPI (blue) in control and UUO kidneys. (n = 3 per group). Scale bar: 20 μm. D Flow chart of the process to collect histone. E Protein expression of H3K9cr, H3K9ac and H3 in fibrotic kidneys of UUO mice were determined by western blotting (n = 5 to 6 animals per group). IHC immunohistochemical, pan-Kcr pan anti-crotonyllysine, IF Immunofluorescence, FAN folic acid nephropathy, UUO unilateral ureteric obstruction, ANCA anti-neutrophil cytoplasmic antibodies, DN diabetic nephropathy, IgAN IgA nephropathy, FSGS focal segmental glomerulosclerosis. Triangle: representative positive staining of pan-Kcr. Statistical analysis by Pearson correlation.

Back to article page