Fig. 4: Integrated analysis of ChIP sequencing and RNA sequencing in kidneys of control and UUO from WT and ACSS2-/- mice. | Nature Communications

Fig. 4: Integrated analysis of ChIP sequencing and RNA sequencing in kidneys of control and UUO from WT and ACSS2-/- mice.

From: Inhibition of ACSS2-mediated histone crotonylation alleviates kidney fibrosis via IL-1β-dependent macrophage activation and tubular cell senescence

Fig. 4: Integrated analysis of ChIP sequencing and RNA sequencing in kidneys of control and UUO from WT and ACSS2-/- mice.The alternative text for this image may have been generated using AI.

A Outline of bioinformatic analysis strategy. B All genes were split into 3 equal groups based on their expression levels calculated from RNA-seq. Mean H3K9cr and H3K9ac ChIP signals are shown for each tertile of gene expression and shown as different colored lines (red line is the gene expressed highest, and blue line is the gene expressed lowest) in both control and UUO mice. C H3K9cr, H3K9ac, and input ChIP signals are shown in both control and UUO mice. TSS, transcriptional start site; TES, transcriptional termination site. D Mean H3K9cr to H3K9ac ChIP ratios in the control and UUO of WT and ACSS2-/- mice. E Comparable analysis from ChIP sequence between UUO of WT and ACSS2-/- mice using GO database, two-sided statistical tests. F Genome browser representation of RNA-seq reads and ChIP-seq reads for Il1b from UUO of control and ACSS2-/- mice. “Gene count” is the number of genes enriched in a GO (gene ontology) or KEGG (Kyoto Encyclopedia of Genes and Genomes) term. ‘Gene ratio’ is the percentage of total differential expression genes in the given GO/KEGG term. UUO unilateral ureteric obstruction, WT wild type, ACSS2-/-: ACSS2 knockout.

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