Fig. 5: The changes of IL-1β expression under different condition. | Nature Communications

Fig. 5: The changes of IL-1β expression under different condition.

From: Inhibition of ACSS2-mediated histone crotonylation alleviates kidney fibrosis via IL-1β-dependent macrophage activation and tubular cell senescence

Fig. 5: The changes of IL-1β expression under different condition.The alternative text for this image may have been generated using AI.

A Schematic overview of process for screening changes of IL-1β both in vivo and in vitro. B Representative IHC staining of IL-1β in kidneys of control and mice treated with UUO or injected with FA (n = 3 per group). Scale bar: upper panels: 100 μm; lower panels:50 μm. C IL-1β concentration of serum and kidney in control mice and UUO of WT and ACSS2-/- mice tested by ELISA kit (n = 3 per group, Control vs UUO: p = 0.039; UUO vs. ACSS2-/- + UUO: p = 0.0042). D IL-1β immunoblotting and quantification of IL-1β immunoblots normalizing to GAPDH (n = 3 per group, Control vs. UUO: p < 0.001; UUO vs. ACSS2-/- + UUO: p < 0.001), as well as IL-1β mRNA levels (n = 5 per group, Control vs. UUO: p < 0.0001;UUO vs ACSS2-/- + UUO: p < 0.0001). in kidneys of control and UUO from WT and ACSS2-/- mice. E IL-1β immunoblotting and quantification of IL-1β immunoblots normalizing to GAPDH (n = 3 per group, Control vs. UUO: p = 0.0342; UUO vs. ACSS2CKO + UUO: p = 0.0315), as well as IL-1β mRNA levels (n = 6 per group, Control vs. UUO: p < 0.001; UUO vs ACSS2CKO + UUO: p < 0.001). in kidneys of control and UUO from WT and ACSS2tecKO mice. F IF colocalization staining of kidney H3k9cr (yellow), IL-1β (red), and DAPI (blue) in UUO of WT and ACSS2-/- mice (n = 3 per group). Scale bar: 25 μm. Ctrl control, UUO unilateral ureteric obstruction, WT wild type, ACSS2-/- ACSS2 knockout, ACSS2 CKO tubular epithelial cell-specific knock out of ACSS2, IHC immunohistochemical, IF immunofluorescence. Data shown are means ± SEM. Statistical analysis by one-way ANOVA with Tukey’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.

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