Fig. 6: H3K9cr-derived IL-1β triggered M1 macrophage polarization and tubular senescence, which could be neutralized by anti-IL-1β IgG. | Nature Communications

Fig. 6: H3K9cr-derived IL-1β triggered M1 macrophage polarization and tubular senescence, which could be neutralized by anti-IL-1β IgG.

From: Inhibition of ACSS2-mediated histone crotonylation alleviates kidney fibrosis via IL-1β-dependent macrophage activation and tubular cell senescence

Fig. 6: H3K9cr-derived IL-1β triggered M1 macrophage polarization and tubular senescence, which could be neutralized by anti-IL-1β IgG.The alternative text for this image may have been generated using AI.

A Schematic overview of process for researching effects of H3K9cr-derived IL-1β on macrophage and tubular cells. B Microscopic images depicted the changes of RAW264.7 cells morphology after stimulation from IL-1β (5 ng/ml) or cellular supernatants collected from HEK-293T cells transfected with ACSS2 plasmids. IL-1β antibody (5 µg/ml) added to neutralize supernatants or IL-1β inverted the morphological changes. Scale bar: 20 μm. C iNOS and IL-1β mRNA levels of RAW264.7 which was stimulated with IL-1β with or without IL-1β antibody neutralization (number of cell wells = 6 per group, control vs IL-1β: p < 0.0001 for iNOS, p < 0.0001 for il1b; IL-1β vs IL-1β+Anti-IL-1β Ab: p = 0.0406 for iNOS, p < 0.0001 for il1b). D iNOS and IL-1β mRNA levels of RAW264.7 which was stimulated with cellular supernatants from HEK-293T cells transfected with ACSS2 plasmids with or without IL-1β antibody neutralization (number of cell wells = 6 per group, control vs supernatants: p = 0.0076 for iNOS, p = 0.0228 for il1b; supernatants vs supernatants+Anti-IL-1β Ab: p = 0.0011 for iNOS, p < 0.0001 for il1b). E P53, IL-1β, Il6, and Mcp1 mRNA levels of TCMK-1 cells which was stimulated with IL-1β (5 µg/ml) with or without IL-1β antibody (5 µg/ml) (number of cell wells = 6 per group, control vs IL-1β: p = 0.0089 for p53, p = 0.0193 for il1b, p < 0.0001 for Mcp1, p < 0.0001 for il6; IL-1β vs IL-1β+Anti-IL-1β Ab: p = 0.0498 for p53, p = 0.0102 for il1b, p < 0.001 for Mcp1, p = 0.002 for il6). F P53, IL-1β, Il6 and Mcp1 mRNA levels of TCMK-1 cells stimulated with cellular supernatants from 293 T cells transfected with ACSS2 plasmids with or without IL-1β antibody neutralization (number of cell wells = 6 per group, control vs supernatants: p = 0.0191 for p53, p < 0.0001 for il1b, p < 0.0001 for Mcp1, p < 0.001 for il6; supernatants vs. supernatants+Anti-IL-1β Ab: p = 0.0068 for p53, p = 0.0367 for il1b, p = 0.0038 for Mcp1, p = 0.025 for il6). ACSS2 OE ACSS2 overexpression. Data shown are means ± SEM. Statistical analysis by one-way ANOVA with Tukey’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.

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