Fig. 4: Removing valves abolishes the mediolateral and expands the longitudinal gradients.
From: Two orthogonal differentiation gradients locally coordinate fruit morphogenesis
a, b Heat maps of averaged area expansion (a) and cell sizes (b) in A. thaliana gynoecium one week after treatment with the NPA. c, d Quantification of cellular growth (c) and cell sizes (d) as a function of the distance from the gynoecium base after NPA treatment (n = 889 cells at 5 DAI; n = 1234 cells at 6 DAI; n = 1460 cells at 7 DAI; n = 1463 cells at 8 DAI; n = 1476 cells at 9 DAI; n = 1478 cells at 10 DAI; three independent time-lapse series). e, f Stomata distribution (e) and quantification of stomatal distribution along the distance from the gynoecium base (f) after NPA treatment (n = 3 stomata at 8 DAI; n = 32 stomata at 9 DAI; n = 125 stomata at 10 DAI; three independent time-lapse series). g–k Expression patterns of PIN1-GFP (g–i) and PIN3-GFP (j–k) in gynoecia after NPA treatment. Heat maps represent the intensity of the PIN1-GFP (i) and PIN3-GFP (k) signals in the L1 layer. Insets indicate membrane localization; arrows indicate plausible orientation of PIN polarization. For plots, the distance was normalized, lines represent the average and shaded areas represent standard deviation (SD). DAI days after organ initiation. Scale bars, 100 µm (a, b, g, h, j) and 50 µm (i, k). See also Supplementary Figs. 5 and 6, Supplementary Movie 4.
