Fig. 6: Removing style identity and reducing CMM expands the longitudinal gradient.
From: Two orthogonal differentiation gradients locally coordinate fruit morphogenesis
a Gynoecium of the crc-1 spt-12 double mutant at 10 DAI (top); longitudinal cut (bottom) shows ovule-like structures. b Heat maps of averaged area expansion of crc-1 spt-12. c Stomata distribution of crc-1 spt-12. d, e Quantification of stomatal distribution as a function of the distance from the valve base (e) or from the medial region (d) of crc-1 spt-12 (n = 6 stomata at 5 DAI; n = 15 stomata at 6 DAI; n = 61 stomata at 7 DAI; n = 309 stomata at 8 DAI; n = 815 stomata at 9 DAI; three independent time-lapse series). f Schematic representation of the influence of the tissue identity and marginal meristematic activity on developmental gradients. Removing valve’s identity (after NPA treatment) abolishes the mediolateral and expands the longitudinal gradients. Removing style identity and reducing CMM (in crc-1 spt-12 mutant), accelerates and expands the longitudinal differentiation gradient. Introducing valve identity and CMM into sepal reorients organ differentiation gradients. Orange indicates CMM; green, cell differentiation status; arrow, the orientation of cell differentiation progression. DAI days after organ initiation. Scale bars, 500 µm (a, b), 100 µm (c–f). See also Supplementary Movie 6.
