Fig. 1: Statins increase circRNA-RBCK1 gene expression in endothelial cells. | Nature Communications

Fig. 1: Statins increase circRNA-RBCK1 gene expression in endothelial cells.

From: Statins improve cardiac endothelial function to prevent heart failure with preserved ejection fraction through upregulating circRNA-RBCK1

Fig. 1

A Cultured human umbilical vein endothelial cells (HUVECs) were treated lovastatin (10 μM) for 24 h. Total RNAs were extracted and subjected to perform RNA sequencing analysis. 13 circRNAs were increased in red and 37 circRNAs were decreased in green. B Cultured HUVECs were treated with lovastatin (10 μM), pravastatin (20 μM), and atorvastatin (10 μM) for 24 h. C Primary human endocardium endothelial cells (EEC), human coronary arterial endothelial cells (CAEC), human myocardial capillary endothelial cells (MCEC), and human aortic endothelial cells (AEC) were incubated with lovastatin (10 μM) for 24 h. The levels of circRNA-RBCK1 were measured by quantitative PCR in (B) and (C). All experiments were repeated from five different donors per cell type. A one-way ANOVA followed by the Dunnett test was used to determine P value in (B). A two-sided unpaired Student’s t test was used to determine P value in (C). Data are presented as mean ± SD. Source data are provided as a Source Data file.

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