Fig. 4: AP-2α is a transcriptional factor of circRNA-RBCK1 in human endocardium endothelial cells (EEC). | Nature Communications

Fig. 4: AP-2α is a transcriptional factor of circRNA-RBCK1 in human endocardium endothelial cells (EEC).

From: Statins improve cardiac endothelial function to prevent heart failure with preserved ejection fraction through upregulating circRNA-RBCK1

Fig. 4

A–C Human EECs were treated with lovastatin (10 μM), pravastatin (20 μM), and atorvastatin (10 μM) for 24 h. The protein levels of phosphorylated AP-2α at serine 219 (pAP-2α) and total AP-2α were measured in (A). The transcriptional activity of AP-2α was assayed by EMSA in (B) and (C). D Human EECs were treated with lovastatin (10 μM, 24 h). Cells were used for the detection of the affinity of AP-2α to RBCK1 gene promoter by ChIP assay. The representative image was obtained from five independent experiments. E and F HEK293 cells transfected with plasmid of luciferase reporter construction containing wildtype (WT) or mutant (MT) RBCK1 promoter were treated with lovastatin (10 μM, 24h) in (E) or co-transfected with AP-2α cDNA in (F). N = 5 per group. A one-way ANOVA followed by Dunnett test was used to determine P value in (A) and (C). A one-way ANOVA followed by Tukey post-hoc tests was used to determine P value in (E) and (F). Data are presented as mean ± SD. Source data are provided) as a Source Data file.

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