Fig. 5: Lovastatin upregulates circRNA-RBCK1 to recouple eNOS in human endocardium endothelial cells (EEC), which is AP-2α dependent. | Nature Communications

Fig. 5: Lovastatin upregulates circRNA-RBCK1 to recouple eNOS in human endocardium endothelial cells (EEC), which is AP-2α dependent.

From: Statins improve cardiac endothelial function to prevent heart failure with preserved ejection fraction through upregulating circRNA-RBCK1

Fig. 5: Lovastatin upregulates circRNA-RBCK1 to recouple eNOS in human endocardium endothelial cells (EEC), which is AP-2α dependent.

Human EECs were infected with lentivirus expressing scramble shRNA or AP-2α shRNA for 48 h followed by incubation with lovastatin (10 μM) for 24 h in presence or absence of ox-LDL (100 μg/ml). Cells were harvested to assay the levels of circRNA-RBCK1 in (A) and GTPCH1 mRNA in (B) by quantitative PCR, BH4 contents by HPLC in (C), ROS productions by DHE/HPLC, and NO levels by DAF/HPLC in (D). N = 5 per group. A one-way ANOVA followed by Tukey post-hoc tests was used to determine the P value in this figure. Data are presented as mean ± SD. Source data are provided as a Source Data file.

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