Fig. 7: Expected outcome depends on acetylcholine signaling.
From: Perirhinal cortex learns a predictive map of the task environment
a Two-photon images of GRAB-Ach3.0 expression in the perirhinal cortex, representing n = 4 animals imaged for 12–42 consecutive sessions, depending on experimental conditions. b Example bulk Ach signals (left) and licking behavior (right) sorted by trial type for one session. Sample (yellow) and test (brown) periods are indicated. Timepoint of stimulus delivery, reward, and the end of the trial are also indicated. c Mean Ach signals across the trial period separated by the choice-aligned beginning of the trial (top). The bottom panel shows a magnified view of signals (dashed rectangle in the top panel) aligned to the behavioral report. d Schematic of GLM depicting basis functions for task variables (top) applied to model Ach signals (bottom). e Example encoding of task factors from imaging session shown in (b). f Encoding of task factors across T1 and T2 sessions. g Schematic of T2 calcium imaging sessions alternating between control no inactivation (Ctl), nAch receptor inactivation by mecamylamine (Mec.), and mAch receptor inactivation by scopolamine (Sco.). h Task performance across pharmacological inactivation sessions. i Stimulus-reward association determined by cross-temporal (CT) decoder performance for Hit vs. non-Hit trials across pharmacological conditions. j Decodable onset timepoint for Stimulus-reward association for (i) across pharmacological conditions. k Correlation between task performance and decoder performance to Expected Hit across pharmacological conditions. l Correlation between task performance and decoder performance to Expected CR across pharmacological conditions. m Correlation between task performance and cross-temporal (CT) performance to Expected Hit across pharmacological conditions. n Correlation between task performance and cross-temporal (CT) performance to Expected CR across pharmacological conditions. Shaded regions = SEM. Error bars = SEM. Scale bar = 20 µm. *P < 0.05, **P < 0.01, ***P < 1×10−4. n = 29 T1, 26 T2 sessions from 4 animals for (f); n = 4 animals, 19 Ctl., 10 Mec., 11 Sco. sessions for (g–n).
