Fig. 1: Karyotype analysis and chromosome-scale assembly of the modern hybrid sugarcane (ZZ1) genome.

a Karyotype analysis, chromosomes with different ancestors of SO (left), SS (right), and recombination between SO and SS (mid) were shown. b overview of sequencing and assembly strategy: (I) assembly, filter, and correct contigs, (II) separate contigs into ROC and YZ groups based on specific k-mer difference, (III) separate contigs into SO and SS groups with mapping quality and similarity, contigs from Rec chromosomes are collected based on Hi-C signals between SO- and SS-derived contigs, (IV) apply haplotype phasing and chromosome-scaffolding in all six groups respectively and Manually adjust. c genomic features of the ZZ1 genome, the reference genome at the center position is Sorghum (Sb), and the collinearity among six chromosome groups in (b) with Sb were drawn respectively.