Fig. 4: Biochemical characterization of DNMT3B-converting mutations. | Nature Communications

Fig. 4: Biochemical characterization of DNMT3B-converting mutations.

From: Structure-guided functional suppression of AML-associated DNMT3A hotspot mutations

Fig. 4: Biochemical characterization of DNMT3B-converting mutations.The alternative text for this image may have been generated using AI.

a DLS plot of MBP-tagged DNMT3A MTase domain, WT, and mutant. b, c Size-exclusion chromatography analysis of MBP-tagged DNMT3A MTase domain examining the mutational effect of DNMT3AR882H (b) or DNMT3AR882C (c) in the absence or presence of additional DNMT3B-converting mutations. d Steady-state enzymatic kinetics of WT, R882H-, R882H/R676K-, and R882H/M674T/R676K-mutated MBP-tagged DNMT3A MTase domain. Data are mean ± SD (n = 3, biological replicates). Source data are provided as a Source Data file.

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