Fig. 3: Effects of Rof and ρ variants.

a Analytical SEC runs, monitoring the interaction between the indicated ρ and Rof variants. For each run, the same fractions were analyzed by SDS-PAGE. First and second panel, SEC runs of isolated ρ and Rof, respectively. Third panel, binding of ρ^wt to Rof^wt. Panels 4–6, binding of indicated Rof variants to ρ^wt. Panels 7–9, binding of indicated ρ variants to Rof^wt. Experiments were performed three times independently with similar results. Source data are provided as a Source Data file. b Effects of Rof variants on ρ-dependent termination in vitro. Assays were done using the λ tR1 template as in Fig. 1c. Termination efficiency values represent means ± SD of three independent experiments. Source data are provided as a Source Data file. c Lag times of growth for IA227 transformed with P_trc plasmids expressing different Rof variants or none. n = 4 biologically independent samples. Source data are provided as a Source Data file. d Production of Rof variants containing a single HA-tag inserted after residue 57 in IA227 cells. Control experiments demonstrated that the expression of HA-tagged Rof is toxic. Rof expression following the induction with 1 mM IPTG was determined by Western blotting with anti-HA antibodies (Millipore Sigma). Experiments were performed three times independently with similar results. Source data are provided as a Source Data file.