Fig. 7: Rof effects on ρ-EC interactions. | Nature Communications

Fig. 7: Rof effects on ρ-EC interactions.

From: Sm-like protein Rof inhibits transcription termination factor ρ by binding site obstruction and conformational insulation

Fig. 7

a Rof interferes with ρ binding to ECs. The ρ6-ADP-Rof5 structure was superimposed on the structure of a ρ/NusA/NusG-modified EC (PDB ID: 6Z9P)33 based on the ρA protomers. RNAP subunits, different shades of gray; NusA, slate blue; NusG, yellow; downstream (d) DNA, brown. Rof sterically interferes with ρ binding to RNAP and Nus factors. b SDS-PAGE analysis of SEC runs, monitoring ρ binding to ECs in the absence and presence of Rof. First panel, NusA/NusG-EC. Second panel, pre-formed NusA/NusG-EC incubated with a three-fold molar excess of ρ hexamer. Third panel, pre-formed NusA/NusG-EC incubated with a three-fold molar excess (relative to ρ hexamer) of ρ-Rof complex. Fourth panel, pre-formed ρ/NusA/NusG-EC incubated with a ten-fold molar excess of Rof (relative to ρ hexamer). Experiments were performed three times independently with similar results. Source data are provided as a Source Data file.

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