Fig. 2: Assessment of species mapping at the individual transcript and cellular levels.
From: Spatiotemporal immune atlas of a clinical-grade gene-edited pig-to-human kidney xenotransplant
Single-cell RNA-sequencing was performed on FACS-enriched immune cells from the xenograft explant as in Fig. 1. Data were aligned to the hybrid human-porcine reference genome. a Species origin of all transcripts for a given immune cell cluster. Table at the bottom enumerates the transcript number for each cluster that mapped to the hg38 (human) or ss11 (pig) portion of the hybrid reference genome. Additional details of pig macrophage transcripts mapping to the hg38 component of the porcine-human hybrid reference genome are given in Supplementary Fig. 4. b The frequency of cells that have low or high levels of transcripts derived from the pig.
