Fig. 3: The transport path of Asc-1.
From: Cryo-EM structure of the human Asc-1 transporter complex
a The substrate interface of Asc-1 in complex with D-Ser (Left panel) and L-Ala (Right panel). Glu257 forms a salt bridge with Arg339 in the occluded conformation. b Oocytes were injected with 10 ng cRNA of human 4F2hc plus 10 ng of human wildtype asc-1, asc-1 mutants. As controls, oocytes were injected with h4F2hc alone (lane 1) or remained non-injected (ni). After incubation of 5 days uptake of 100 μM [14C]glycine was determined over a time period of 40 min. Data are presented as mean ± SD, which was calculated from the uptake activity of independent oocytes (in the order as shown in Fig. 3b n = 12, 13, 13, 24, 23, 26, 28, 11, 26, 28, 24, 26) derived from two independent batches.
