Fig. 1: DRI is linked to heme homeostasis.

a Phylogenetic tree of the DRI-containing protein family. Background color shading of clades is blue for prokaryotes and green for eukaryotes, while circles at the tips of leaves indicate phylum as given in the color key. Inner circles display information about the protein represented by each leaf according to the color key. The outermost circle indicates whether the protein contains TMEM254 (purple), SIP (light green), HO (teal), or the PNPO-like domain at either the N-terminus (blue) or C-terminus (yellow). The second outermost circle indicates whether the protein is less than 150 amino acids in length (grey). The third circle indicates whether a gene encoding a protein predicted to be involved in Fe homeostasis or Fe binding is found near the gene encoding the DUF2470-containg protein (greyish blue). The innermost circle indicates whether the gene encoding the DUF2470 protein is near a gene encoding either HO (teal) or SIP (light green). b Cartoon representation of the domain structure of selected DRI-containing proteins. Numbers to the left correspond to numbered clades in panel a. Coloring is used to indicate distinct and shared domains: DUF2470 (dark green), TMEM254 (purple), heme oxygenase (Pfam domain PF01126; teal), the PNPO-like split barrel domain (dark blue), and the SIP domain (light green). c HMMER alignment and WebLogo representation showing the conservation of DRI sequence in each clade. Source data are provided as Supplementary Data 1.