Fig. 6: Plasma HRG exhibits prothrombotic roles in the absence of FXII.

a The activity of antithrombin was measured in WT and Hrg^−/− plasma on endothelial cell surface, or in the presence or absence of heparin. The number of independent samples (n value) analyzed in each group was indicated above the bars. b Immunoblotting of HRG antigen in equal amounts of plasma from F12^−/− mice treated with control or vivo-siRNA to HRG, with transferrin as the loading control. c Representative images of platelet accumulation (Red) and fibrin generation (Green), as visualized by Dylight-649-congugated anti-CD42c and Alexa-488-conjugated 59D8 antibody, respectively, at indicated time points following laser injury in the cremaster arterioles in F12^−/− mice treated with control or vivo-siRNA to HRG (scale bar: 30 μm) (Supplementary Movie 4). The median fluorescence intensity of platelets (d) and fibrin (e) were calculated and plotted over time. The area under the curve (AUC) for platelets (f) and fibrin (g) were analyzed from each individual thrombus in the two groups. The number of thrombi (n value) analyzed in each group was indicated above the bars. Ctrl, control. Data are presented as mean values ± SEM and analyzed by two-tailed Welch’s t-test (a) or two-tailed Mann–Whitney U-test (f and g). Source data are provided as a Source Data file.